FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27991226
Sequences flagged as poor quality0
Sequence length65
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT645110.23046864756834873No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATAAACAATGCACTAAA326020.1164722116851902No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC310890.11106694647815712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT190050.027.0022181
CTACACG202400.024.92225557
CCGGCTT225850.024.5176282
AACGCTA210550.024.40588823
ACGCTAC212050.024.21933424
GCACTAA100100.023.92895358
CGCTACC214750.023.83241525
GTTCCTA78250.023.67458715
TTCACGC220950.023.4440697
ACACGTT216650.023.3976159
TCACGCT222600.023.3233018
TACACGT217550.023.25448458
AAAACGC225400.022.85034821
GCTCTAC226600.022.32610354
GAAAACG229650.022.3247120
GCCCCGT51750.022.2393471
TGCACTA109350.022.22849857
CGGCTTC257800.022.1195093
AAACGCT236600.021.75616822
ACGCTCA239250.021.73731810
Produced by FastQC (version 0.11.5)