FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22935711
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16400.026.80058748
CGTATGC17650.025.57088546
CTCGTAT17500.025.11581244
TCGTATG17650.025.06949445
TCTCGTA20750.021.89281843
GATCGGA21150.021.4881861
ATCGGAA22500.020.5919282
ATCTCGT26050.017.7783342
ATGCCGT25250.017.64095949
ACACGTC28750.016.00611113
CGTCTGA29600.015.94510216
TGCCGTC33800.013.00397750
CATCTCG35800.012.93646641
TCGGAAG36750.012.8447633
GTATGCC38500.012.4123347
CCGTCTT39450.011.81469152
ACGTCTG41650.011.75688215
GCCGTCT38800.011.632318551
CGGAAGA42900.011.3471614
CGTCTTC40950.011.16580653
Produced by FastQC (version 0.11.5)