FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26529854
Sequences flagged as poor quality0
Sequence length65
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTATGCCGTCTTCTGCTTG434480.1637702190143979TruSeq Adapter, Index 5 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG59750.048.1864248
CGTATGC61000.047.19899446
TCGTATG61000.047.1506345
CTCGTAT61500.046.67136444
GATCGGA62750.045.67941
TCTCGTA63700.044.9668643
ATCGGAA66900.042.9900862
ATGCCGT68100.042.2325549
ATCTCGT68550.041.871742
ACACGTC72450.039.8597113
CGTCTGA72450.039.57545516
TGCCGTC75250.038.3373650
TCGGAAG79050.036.4199563
ACGTCTG80600.035.976315
GCCGTCT80850.035.8287151
CGGAAGA84600.034.1353074
CCGTCTT84450.034.0568652
CGTCTTC84750.033.93630653
GTATGCC88350.032.8549947
CACGTCT89250.032.7863714
Produced by FastQC (version 0.11.5)