FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22186317
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCAATCTCGTATGCCGTCTTCTGCTTG438940.19784266131237555TruSeq Adapter, Index 9 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA52600.051.3323941
TATGCCG53250.050.51685348
CGTATGC55200.048.78572546
CTCGTAT55500.048.6814644
TCTCGTA56450.048.01894843
ATCGGAA56750.047.8915022
ATGCCGT56750.047.45325549
TGCCGTC57800.046.48925850
CGTCTGA58600.046.30780816
GCCGTCT58500.046.08423651
TCGTATG58750.046.0386545
ACACGTC59400.045.8824513
ATCTCGT60200.045.07673642
TCGGAAG61650.043.8935783
ACGTCTG64300.042.38625315
CGGAAGA64100.042.2619364
CGTCTTC65750.041.13728753
CCGTCTT66500.040.6289852
CACGTCT70400.038.79737514
AATCTCG72000.037.9759241
Produced by FastQC (version 0.11.5)