FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31333514
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1449230.4625175459094693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG289650.035.50013
AGACCGC288300.035.4900254
GACCGCG295150.034.4963575
TTCGACG45150.033.5769236
ACCGCGT304550.033.527726
TCAGACC323250.032.978082
CCGCGTT318300.032.051487
CGCGTTC318000.032.0446178
CTCAGAC352850.031.3196581
GTTCGAC52700.028.2075375
GCGTTCT375050.027.2724029
CGGTATA75150.027.0423120
CGACGAG56150.026.5262438
TCGACGA57650.026.3988237
CGTTCTC404600.025.93662810
AATACGG421000.023.95255732
TACGGAG420200.023.85773834
ATACGGA423800.023.78734433
CCTCTCA445650.023.32356319
CAATACG440250.023.09284831
Produced by FastQC (version 0.11.5)