FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23182939
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTATGCCGTCTTCTGCTTG516300.22270687939954462TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA62450.052.4008181
TATGCCG62000.052.28353548
CTCGTAT64400.050.4725944
CGTATGC65650.049.55639346
TCTCGTA65700.049.51878743
ATCGGAA67450.048.546442
GCCGTCT67100.048.39758751
TCGTATG67550.048.2061745
TGCCGTC67600.048.08325250
ATGCCGT68850.047.25311749
CGTCTGA69100.047.12484716
ACACGTC69750.046.55882313
ATCTCGT70050.046.4437442
TCGGAAG70950.046.2171943
CGGAAGA75350.043.635894
ACGTCTG74850.043.58351515
CGTCTTC75000.043.4963553
CCGTCTT75000.043.2997152
CACGTCT79200.041.22696314
AATCTCG81200.040.02998441
Produced by FastQC (version 0.11.5)