FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29649884
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTATGCCGTCTTCTGCTTG778630.26260811003510165TruSeq Adapter, Index 5 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG93950.051.52217548
CGTATGC95500.050.74772646
CTCGTAT96100.050.308144
GATCGGA97350.049.8167721
TCGTATG98400.049.52190845
TCTCGTA97600.049.50470443
ATGCCGT100750.048.19097549
ATCGGAA102150.047.876372
ATCTCGT102350.047.26453842
CGTCTGA104200.046.93446416
TGCCGTC104800.046.2998650
ACACGTC107300.045.71816613
GCCGTCT107800.045.0660951
TCGGAAG109500.044.635813
CATCTCG110800.043.68645541
CGTCTTC113950.043.0490253
CGGAAGA113600.043.0261424
CCGTCTT113600.042.8440952
ACGTCTG116650.042.1021815
CACGTCT121500.040.5430814
Produced by FastQC (version 0.11.5)