FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13366895
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTATGCCGTCTTCTGCTTG446770.3342361857409668TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG47650.053.85348548
GATCGGA49250.053.2718161
TCTCGTA49800.052.23922343
CGTATGC49750.052.05457746
CTCGTAT50000.051.85329444
ATGCCGT51100.050.4484749
ATCGGAA52500.050.3084032
ATCTCGT51800.050.22226742
TCGTATG52350.049.8636545
TGCCGTC51800.049.65303450
ACACGTC53600.049.41616413
GCCGTCT52200.049.3290651
TCGGAAG53700.049.0193373
CGTCTGA54500.048.32987616
CGTCTTC55850.046.42210453
CCGTCTT55800.046.199452
ACGTCTG58150.045.49916515
CGGAAGA58150.045.3695644
CACGTCT59750.044.37951314
AATCTCG59050.044.0061641
Produced by FastQC (version 0.11.5)