FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23310807
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTTCTAATCTCGTATGCCGTCTTCTGCTTG817870.3508544341686669TruSeq Adapter, Index 15 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA95350.054.07121
TATGCCG95300.053.60585848
CTCGTAT96850.052.5656644
CGTATGC97500.052.42653746
TCTCGTA98050.051.95241543
TCGTATG99200.051.55783545
ATCGGAA100650.051.339792
ATGCCGT101550.050.33556749
GTCACGT101950.050.16916329
TGCCGTC102050.050.08894350
ACACGTC102650.050.08350813
ATCTCGT102350.049.74093242
GCCGTCT103150.049.5833951
AGTCACG103650.049.4886128
CGTGTTC103650.049.3467433
TCGGAAG104750.049.3366743
CGTCTGA105800.048.39721716
CGGAAGA107650.048.1172524
CGTCTTC108750.047.19286753
CCGTCTT109050.046.92779552
Produced by FastQC (version 0.11.5)