FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29546554
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC554810.18777485861803037No Hit
CTCAGACCACGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC354880.12010876124505078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC124350.029.7086874
GACCGCG124400.029.3845165
ACCGCGT125350.029.250916
TCAGACC216900.028.7997322
CAGACCG130850.028.4133033
CCGCGTT130450.028.084347
CGCGTTC130500.028.0737238
CTCAGAC244250.026.7301391
AATACGG226700.026.46730432
TACGGAG229350.025.99291834
ATACGGA231600.025.90636433
GAACGAT242800.024.58951645
CAATACG246950.024.356731
AACGATC249250.024.13071846
ACGGAGA252050.023.6285535
AAGAACG255300.023.5125843
CCCAATA261300.023.33570329
AGAACGA259550.023.15034544
CGTTCTC267750.023.0363410
CGGAGAG257050.023.03123536
Produced by FastQC (version 0.11.5)