FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30231358
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT306730.10146087383835023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG83350.026.33177857
AACGCTA84650.025.9619823
ACGCTAC86050.025.57387224
CGCTACC88250.024.9029125
AAAACGC98000.022.57586921
TACACGT97650.022.53613558
GCCCTAA72300.021.33896652
ACACGTT106150.020.6245959
GAAAACG109700.020.1680520
AAACGCT110300.020.16531822
CCTAAGC78550.019.94148854
CCGGCTT129050.019.9340272
GTTCCTA69900.019.91923315
GCCGGCT128400.019.4174821
TCACGCT119850.019.3706328
GCTCTAC111250.019.35673554
GCACTAC72150.019.05277325
GTCCGTT41700.018.8919331
TTCGGTG117750.018.6387948
TTTCGGT119200.018.5110547
Produced by FastQC (version 0.11.5)