FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19538781
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1342210.6869466421676972No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA267710.13701468888975213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG271550.035.2965353
AGACCGC274750.034.863964
GACCGCG275700.034.422825
CGCGTTC281100.033.959828
TCAGACC292300.033.921252
ACCGCGT282950.033.6141326
CCGCGTT285450.033.44237
CTCAGAC305700.033.371361
AATACGG314500.029.75265932
GCGTTCT323400.029.6547789
ATACGGA315850.029.6535133
TACGGAG312200.029.6508934
CGTTCTC338000.029.22914710
CAATACG334000.028.29823331
CCCAATA338950.028.250529
GAACGAT337050.028.19991345
ACGGAGA330600.028.05416735
AACGATC345500.027.70667346
CCCCAAT346000.027.64077428
AGAACGA346550.027.4694344
Produced by FastQC (version 0.11.5)