FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12964190
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC375320.2895051676965549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG87950.031.5307773
AGACCGC87850.031.3315984
GACCGCG87400.031.290395
ACCGCGT88050.031.2269276
CGCGTTC88950.030.9417518
CCGCGTT89150.030.8061567
TCAGACC107000.026.9929282
CTCAGAC116900.026.004371
GCGTTCT109100.025.2811039
TTCGACG10250.024.4645796
AATACGG112950.024.1595132
TACGGAG113950.023.7662734
ATACGGA114850.023.7084633
CAATACG120000.023.01056331
CGTTCTC125900.022.7278610
GAACGAT124450.022.21121445
ACGGAGA123850.021.9379635
CCCAATA131000.021.46005229
AACGATC130100.021.40534846
AAGAACG130650.021.1345243
Produced by FastQC (version 0.11.5)