FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25012900
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT404640.16177252537690553No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC302780.1210495384381659No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT280450.11212214497319382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG76100.026.05375557
AACGCTA78650.025.46857823
ACGCTAC81650.024.5336424
CGCTACC81700.024.41029525
GCCCTAA95100.023.82102852
GTTCCTA53500.023.54266415
CCTAAGC98100.023.21287554
TACACGT86850.022.93082458
AAAACGC90900.022.29468521
ACACGTT92950.021.23772659
CCCTAAG107600.021.21823953
CTAAGCT111100.020.65608255
GAAAACG98350.020.5146120
GCTCTAC98900.020.48996254
AAACGCT99500.020.42818522
CCGGCTT120050.020.345482
GCCCTAT62950.020.14899453
GCACTAC65000.019.92505625
CCTACTC63400.019.63385818
TTCGGTG101900.019.39454348
Produced by FastQC (version 0.11.5)