FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20385853
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT240980.1182094269001155No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT237860.11667895378231169No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC221070.10844285004900213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA69750.026.09455923
CTACACG70500.025.81708557
ACGCTAC70850.025.77269424
CGCTACC71650.025.4437625
AAAACGC79500.022.96855221
GCCCTAA76000.022.82300252
CCTAAGC77050.022.62683954
TACACGT79900.022.5582658
ACACGTT80450.022.4746159
CCCTAAG79850.021.9442453
GCTCTAC83950.021.6807154
GTTCCTA45050.021.60878415
GAAAACG85750.021.43206620
GCCGGCT89600.021.3393141
AAACGCT88400.020.72284722
CCGGCTT102050.020.6987022
CGTGGTT91300.019.80589541
AGCCCTA93850.019.8020951
TCGGTGC90550.019.48128149
CTAAGCT89950.019.48024255
Produced by FastQC (version 0.11.5)