FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20823654
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTATGCCGTCTTCTGCTTG343230.16482697993349293TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41050.050.00930848
GATCGGA42400.048.786311
CTCGTAT42950.047.865744
TCTCGTA43850.046.6814843
CGTATGC44450.046.1840846
ATCGGAA45900.045.344822
ATGCCGT45650.045.22848549
ACACGTC46550.044.8610513
TGCCGTC45500.044.66451350
TCGTATG47350.043.6669545
GCCGTCT47350.042.9194451
ATCTCGT49450.041.514342
CGTCTGA50200.041.4816316
TCGGAAG49950.041.422143
CCGTCTT52650.038.7110152
CGTCTTC52600.038.6917353
CGGAAGA54050.038.662294
ACGTCTG55200.037.83119215
CACGTCT58400.035.85926414
AATCTCG58950.034.82412341
Produced by FastQC (version 0.11.5)