FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36085080
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1870190.5182723718500832No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA415870.11524707718536303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC369050.034.7693984
CAGACCG370600.034.7517663
GACCGCG370900.034.48455
ACCGCGT374550.034.280396
CCGCGTT392550.032.6922457
TCAGACC413900.032.300922
CGCGTTC397850.032.29388
CTCAGAC446900.030.7833751
TTCGACG31650.030.0088316
GCGTTCT446050.028.8967239
CGTTCTC502800.026.5152810
AATACGG501300.025.6890932
TACGGAG501850.025.45571934
ATACGGA510050.025.27730433
CCTCTCA540700.024.53707319
CAATACG533500.024.48124531
GAACGAT533650.024.29718845
CCCAATA543800.024.10443529
ACGGAGA535100.023.89012535
AACGATC544950.023.8474946
Produced by FastQC (version 0.11.5)