FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21609062
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTATGCCGTCTTCTGCTTG3330041.5410386623908061TruSeq Adapter, Index 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA361150.057.5410541
ATCGGAA417500.049.8370132
TATGCCG409600.049.82116348
CGTCTGA416800.049.66824716
CTCGTAT409250.049.64753344
ACACGTC417850.049.5787413
TCTCGTA410550.049.5765443
TCGGAAG421300.049.4061433
CGTATGC413900.049.3819646
TCGTATG414400.049.35085745
ATGCCGT415200.049.1847249
CGGAAGA424200.049.0037354
TGCCGTC416750.048.99471350
GCCGTCT418300.048.93304451
ATCTCGT416000.048.9270442
ACGTCTG424250.048.78215415
CGTCTTC422600.048.4352653
CCGTCTT423000.048.3964352
CACGTCT428450.048.37279514
GCACACG436850.047.5236911
Produced by FastQC (version 0.11.5)