FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22162976
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTATGCCGTCTTCTGCTTG13609406.140601334405632TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1462500.058.699011
ATCGGAA1473500.058.4337542
ACACGTC1481850.058.30644213
TCGGAAG1474150.058.302863
CGTCTGA1479200.058.19753616
CGGAAGA1476800.058.128334
ACGTCTG1484650.057.9958215
TATGCCG1481550.057.94190648
CTCGTAT1472650.057.9398844
CACGTCT1491150.057.90719214
TCTCGTA1474400.057.8695943
CGTATGC1485200.057.83701346
GCACACG1496100.057.7984111
TCGTATG1486850.057.79520445
ATCTCGT1477450.057.7361542
TGCCGTC1487250.057.7128250
GCCGTCT1487650.057.69531651
ATGCCGT1488000.057.68967449
CACACGT1499400.057.67513312
CCGTCTT1493800.057.48129352
Produced by FastQC (version 0.11.5)