FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30467761
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1805470.5925837477850768No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA408960.13422712617445043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG354800.034.6219753
AGACCGC356200.034.4030764
GACCGCG361950.033.750595
ACCGCGT372600.032.913066
TCAGACC389250.032.4520952
CCGCGTT383650.032.0795367
CGCGTTC386900.031.8634388
CTCAGAC413750.031.2909771
GCGTTCT428600.028.8941089
AATACGG434550.028.34894232
TACGGAG433350.028.1483934
ATACGGA437250.028.13341133
CAATACG455000.027.191531
GAACGAT459450.026.82562645
CGTTCTC475600.026.67138710
AACGATC464000.026.6643446
ACGGAGA460300.026.58369635
CCCAATA468300.026.46942129
AAGAACG473400.026.04132543
CGGAGAG471850.025.83919736
Produced by FastQC (version 0.11.5)