FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23242102
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTCTTCTGCTTG444090.19107135834788092TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG56400.050.31075348
GATCGGA57750.049.3591351
CTCGTAT58450.048.64714444
CGTATGC58800.048.2572546
TCTCGTA59250.047.94052543
TGCCGTC60850.046.5346550
TCGTATG61600.046.20739445
ATGCCGT61700.045.98918549
ATCGGAA62200.045.9637072
GCCGTCT63750.044.6953951
ATCTCGT63700.044.49904342
ACACGTC64350.044.46190313
CGTCTGA65150.043.64447816
TCGGAAG65700.043.4215553
ACGTCTG69100.041.2349815
CGTCTTC69350.041.2138453
CGGAAGA69900.040.9813584
CCGTCTT70450.040.69593452
CACGTCT73400.039.30153314
GCACACG77350.037.2943811
Produced by FastQC (version 0.11.5)