FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23360856
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTATGCCGTCTTCTGCTTG20682178.853344243892433TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2194300.058.7736051
ATCGGAA2202850.058.545532
TCGGAAG2208350.058.456863
ACACGTC2207900.058.41549313
CGTCTGA2207050.058.361116
CGGAAGA2212250.058.3111274
CGATAGA2187500.058.25718333
ACGATAG2190150.058.24865332
GTCACGA2194700.058.22600629
CTCGTAT2203150.058.19913544
TATGCCG2209800.058.19194448
TCTCGTA2203450.058.17514843
ACGTCTG2215150.058.15834815
CACGATA2194400.058.15600231
CGTATGC2211250.058.1513746
AGTCACG2204950.058.13265228
TCGTATG2213850.058.11638645
CACGTCT2219200.058.09146514
TGCCGTC2214100.058.0829350
ATGCCGT2214000.058.08155449
Produced by FastQC (version 0.11.5)