FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21869181
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGTATGCCGTCTTCTGCTTG435480.19912954216255285TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG56450.051.20533848
CTCGTAT56950.050.65242844
CGTATGC57450.050.262746
GATCGGA58150.050.156081
TCTCGTA57550.050.12433643
TGCCGTC59750.047.83425550
ATCGGAA61700.047.3191342
TCGTATG61150.047.26968445
GCCGTCT60900.047.02784351
ATGCCGT61950.046.99254249
ATCTCGT62650.046.37354742
TCGGAAG63650.045.7670563
ACACGTC64350.045.56117613
CGTCTGA65400.044.60378316
CGTCTTC66950.042.86623853
CGGAAGA68050.042.721094
CCGTCTT68800.041.79932852
ACGTCTG70100.041.6977615
CACGTCT73050.040.13500214
AATCTCG73950.039.1278541
Produced by FastQC (version 0.11.5)