FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26120266
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCTCGTATGCCGTCTTCTGCTTG428277316.396360588364605TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4540200.058.8999521
ATCGGAA4553850.058.7268452
TCGGAAG4557800.058.688983
CGGAAGA4560100.058.621864
ACACGTC4560000.058.6078213
CGTCTGA4559500.058.5408316
ACGTCTG4564900.058.48967415
CACGTCT4570150.058.47184814
GCACACG4571650.058.44413811
CACACGT4574850.058.40905812
TCTCGTA4551300.058.2882843
CTCGTAT4551750.058.28316544
TATGCCG4563750.058.26995548
CGTATGC4568550.058.2431846
TCGTATG4572450.058.22704745
ATCTCGT4555400.058.21639342
ATGCCGT4569400.058.19467549
TGCCGTC4569550.058.17791750
GCCGTCT4570000.058.17347751
AATCTCG4549850.058.13828341
Produced by FastQC (version 0.11.5)