FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27074963
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC605550.22365681533895354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC137500.032.5416074
GACCGCG138500.032.0297935
CCGCGTT141500.031.5159057
CAGACCG142800.031.439373
ACCGCGT141750.031.398426
CGCGTTC144100.030.98828
TTCGACG24900.028.783836
AATACGG167100.026.98762732
TCAGACC176550.026.7492122
TACGGAG167450.026.68462634
ATACGGA171050.026.41613833
GAACGAT180750.025.0474645
GCGTTCT180850.024.723759
AACGATC186450.024.45573646
CAATACG185750.024.3892531
GTTCGAC28950.024.0447085
CTCAGAC208050.023.9034611
ACGGAGA189950.023.5858835
CGGTATA39750.023.29934920
AAGAACG194450.023.26761243
Produced by FastQC (version 0.11.5)