FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26796578
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGTCAATCTCGTATGCCGTCTTCTGCTTG667650.24915494806836905TruSeq Adapter, Index 18 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG85600.051.72377448
CTCGTAT87350.050.72129444
CGTATGC87700.050.7206846
TCTCGTA88200.049.93148843
GATCGGA89250.049.8351971
TCGTATG89700.049.7870945
ATGCCGT91850.048.1076749
ATCGGAA95100.047.300282
TGCCGTC94950.046.56747450
ATCTCGT94650.046.52860642
GTCACGT94900.045.6718229
GCCGTCT97700.045.40767351
CGTCTGA98050.045.12508816
ACACGTC99300.044.94505313
TCGGAAG100450.044.7223243
AGTCACG98700.044.1525828
TCACGTC101100.043.4254730
CCGTCTT103750.042.70366352
CGTCTTC105050.042.48407453
CGGAAGA106600.042.0329174
Produced by FastQC (version 0.11.5)