FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13950420
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC685370.49128986797530116No Hit
CTCAGACCACGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC326940.23435853544194368No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA152870.1095809301798799No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT145240.10411156079888635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAC219650.035.5098461
TCAGACC215150.035.275282
AGACCGC152850.033.074444
CAGACCG153700.033.0660173
GACCGCG153650.032.7102745
ACCGCGT155550.032.3669136
GACCACG79600.030.7547475
CCGCGTT168500.029.844047
CGCGTTC169550.029.6418258
CGTTCTC261300.028.55710810
ACCACGT89150.027.6581256
GCGTTCT190250.026.4631799
ACGTTCT96250.025.495049
AGACCAC100700.024.8378224
CCTCTCA305200.024.51885419
GTTCTCT313250.024.1977611
CCACGTT102000.024.057827
CAGACCA107600.023.5204433
CTCTCCC322200.023.43431314
CTCCCTC324650.023.22112516
Produced by FastQC (version 0.11.5)