FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19145839
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTATGCCGTCTTCTGCTTG289180.15104065170505196TruSeq Adapter, Index 14 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAT40500.050.10845637
TATGCCG41550.049.55027848
GATCGGA42500.049.4716531
CGTATGC43250.047.80723646
CTCGTAT43500.047.4646744
TCTCGTA43500.047.39723643
ATCGGAA45250.046.7499732
CAGATCG43800.045.5998733
ATGCCGT45750.045.3882349
ATCGCAA45250.044.71807536
TGCCGTC46500.044.4024350
TCGTATG46850.044.19663645
GCCGTCT46850.044.0707251
TCGGAAG48550.043.6903273
ATCTCGT47350.043.48132742
ACACGTC48000.043.32210513
CGTCTGA49750.041.976316
CGCAATC49450.041.51637338
GATCGCA49600.040.9164535
CGTCTTC52400.039.96582453
Produced by FastQC (version 0.11.5)