FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23575830
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATCTCGTATGCCGTCTTCTGCTTG359450.1524654699325538TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA45750.047.74711
TATGCCG51650.041.57700748
CTCGTAT51750.041.2116644
CGTATGC52800.040.44797546
TCTCGTA54500.039.40279843
ATCGGAA56250.039.0374262
ATGCCGT56350.037.8997949
TCGTATG56900.037.68897245
TGCCGTC57000.037.62285250
GCCGTCT57950.037.311551
ACACGTC59550.036.8538913
TCGGAAG59700.036.8235863
ATCTCGT60400.035.45617342
CGTCTGA62000.035.3024116
ACGTCTG65350.033.76355415
CGTCTTC65300.033.0214753
CCGTCTT66550.032.62285252
CGGAAGA67950.032.3079344
CACGTCT70250.031.53448914
AATCTCG74150.029.15983241
Produced by FastQC (version 0.11.5)