FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14252397
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC429180.30112829441952815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC99250.034.3900224
CAGACCG99500.034.3332673
GACCGCG100550.033.6520085
ACCGCGT102250.033.1504486
CGCGTTC103450.032.7645268
CCGCGTT104800.032.314327
TTCGACG15400.030.4585386
TCAGACC120050.029.7093942
CTCAGAC134800.027.9859711
AATACGG124750.026.81556332
TACGGAG124000.026.45446834
ATACGGA127300.026.2320633
GCGTTCT132500.025.6701169
CGTTCTC142450.024.9953510
GAACGAT137450.024.7460945
ACGGAGA135350.024.27967535
CCCAATA145750.023.90301529
CAATACG142800.023.88053331
AACGATC146300.023.61209946
CGGAGAG140350.023.07840736
Produced by FastQC (version 0.11.5)