FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20981691
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31550.035.06138248
CTCGTAT32550.034.256144
GATCGGA33500.033.5553781
TCGTATG33350.033.52281645
TCTCGTA33850.033.1147943
CGTATGC33850.032.94050646
ATGCCGT39250.028.63402749
ATCTCGT39100.028.59298942
ATCGGAA41000.027.4847742
ACACGTC44600.025.19922613
CGTCTGA45100.025.11607716
TGCCGTC45600.024.9053750
CATCTCG49400.022.75072341
TCGGAAG50900.022.1985553
GCCGTCT52200.021.75672751
GTATGCC52350.021.46868547
CGGAAGA53800.021.2761554
ACGTCTG54250.021.09740815
CCGTCTT55350.020.46524452
CACGTCT57950.019.9539914
Produced by FastQC (version 0.11.5)