FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29675450
Sequences flagged as poor quality0
Sequence length65
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTCTTCTGCTTG1633290.5503842401715897TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA198150.055.546231
CTCGTAT198550.055.0175144
TATGCCG200400.054.86290448
CGTATGC200750.054.8407346
TCTCGTA199300.054.72166443
TCGTATG203200.054.1940345
ATCGGAA204700.053.951522
ATGCCGT207400.053.03582449
ATCTCGT207600.052.4633342
ACACGTC210650.052.00751513
TCGGAAG214700.051.3838543
TGCCGTC214900.051.36330850
CGTCTGA212550.051.29428516
GCCGTCT219600.050.1853651
CGGAAGA220100.050.042784
CCGTCTT221600.049.79897752
ACGTCTG220050.049.47898515
CGTCTTC223100.049.29226753
CACGTCT229700.047.83556714
GTATGCC231450.047.73223547
Produced by FastQC (version 0.11.5)