FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19193362
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTATGCCGTCTTCTGCTTG391150.20379441600695075TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG48500.052.6671448
CTCGTAT49450.051.47638744
GATCGGA50300.051.1032521
TCTCGTA51700.049.35022443
CGTATGC51850.049.32122846
ATGCCGT53150.048.2258749
TCGTATG53500.048.18603545
TGCCGTC53050.048.14997550
GCCGTCT53550.047.64531351
GTCACGG54200.046.91493629
ATCGGAA55400.046.3937842
ATCTCGT55450.046.1191342
ACACGTC55750.046.02967513
TCGGAAG56450.045.4262853
CGTCTGA57300.044.88773316
TCACGGA56800.044.87280730
AGTCACG57350.044.38767628
CGGAGAA58800.043.39669433
CGGAAGA59300.043.2928284
ACGGAGA59350.043.14364632
Produced by FastQC (version 0.11.5)