FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25834817
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTCTGCTTG444670.1721204373152711TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA59400.051.183921
TATGCCG63400.047.13148
CGTATGC65800.045.63519746
CTCGTAT65650.045.200344
TCTCGTA66000.044.87121243
ATCGGAA69000.044.2598272
TCGTATG69150.043.4670445
TGCCGTC69050.043.23221650
ATGCCGT69500.043.03717849
TCGGAAG72150.042.320443
ACACGTC71750.042.3041813
GCCGTCT71050.042.13981251
ATCTCGT70650.042.0849142
CGTCTGA72400.041.8417616
CGGAAGA76650.039.7973064
CATCTCG75750.039.25147241
CCGTCTT78450.038.3909252
CGTCTTC78650.038.1432753
ACGTCTG79750.038.13344215
CACGTCT82950.036.6979114
Produced by FastQC (version 0.11.5)