FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21009109
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCAATCTCGTATGCCGTCTTCTGCTTG1168300.5560921217553776TruSeq Adapter, Index 9 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA143750.054.747051
TATGCCG154600.049.5129648
CTCGTAT156000.048.69043444
ATCGGAA162350.048.6486632
TCTCGTA156700.048.64234543
CGTATGC158550.048.3724546
TCGTATG159950.047.93062245
ATGCCGT160350.047.8294549
TCGGAAG165550.047.717163
ACACGTC162500.047.5959913
CGTCTGA162350.047.56728416
TGCCGTC162350.047.27657350
GCCGTCT163100.047.18577651
ATCTCGT161850.047.1674742
CGGAAGA169300.046.571854
ACGTCTG168650.045.8953315
CGTCTTC168400.045.64816353
CCGTCTT169700.045.43753452
CACGTCT174150.044.54749314
GCACACG180100.043.22317511
Produced by FastQC (version 0.11.5)