FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20750667
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACCAAATCTCGTATGCCGTCTTCTGCTTG2407461.160184393108906TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA272150.057.08781
TATGCCG277600.056.56959548
CTCGTAT277300.056.33770444
TCTCGTA278400.056.14892243
CGTATGC280200.056.08975646
ATCGGAA278900.055.983482
TCGTATG281850.055.7718645
TGCCGTC281650.055.74567450
CGTCTGA282100.055.60446516
TCGGAAG280100.055.5535053
ACACGTC285400.055.45760313
ATCTCGT282500.055.3662842
ATGCCGT283800.055.33375549
GCCGTCT284150.055.25481451
CGGAAGA283250.054.899694
CGTCTTC288850.054.38585753
ACGTCTG288900.054.3671415
CCGTCTT290400.054.1260552
CACGTCT293850.053.83274514
AATCTCG295900.052.95881341
Produced by FastQC (version 0.11.5)