FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24154568
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17700.024.66552448
GATCGGA17750.024.1094021
CGTATGC19050.023.07237246
CTCGTAT20150.021.5200544
TCGTATG20700.021.09076545
TCTCGTA21350.020.7249943
ATGCCGT24250.018.24672949
ATCGGAA24550.018.0317062
ATCTCGT26200.017.56403442
CGTCTGA26300.016.93647816
ACACGTC26900.016.33935513
TGCCGTC27550.016.16816750
GCCGTCT29100.015.40834851
CATCTCG31750.014.30796841
ACGTCTG32700.013.98250215
TCGGAAG32300.013.7007843
CCGTCTT34750.012.98826352
CGGAAGA35100.012.7758184
CGTCTTC36800.012.1845753
CACGTCT39650.011.68039214
Produced by FastQC (version 0.11.5)