FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19679630
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTATGCCGTCTTCTGCTTG828500.42099368738131765TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG92400.054.07580648
CTCGTAT93850.053.58603744
GATCGGA94800.053.259431
CGTATGC94700.052.76245546
TCTCGTA95550.052.6943943
ACGTATC96300.052.32127834
CGTATCA96400.052.20579535
TCGTATG97800.051.63289345
ATCGGAA99100.050.9489752
TGCCGTC98700.050.71381450
ATCTCGT99300.050.64501642
ATGCCGT99000.050.58992449
GCCGTCT99750.050.2391251
ACACGTC101900.049.75803413
TCGGAAG103050.048.9387863
CCGTCTT103500.048.5043652
CGTCTGA104450.048.4872816
CGTCTTC105250.048.03417253
TCACACG105200.047.9789830
CACGTAT106650.047.43730533
Produced by FastQC (version 0.11.5)