FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12643233
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT167030.13211019681437494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC32150.024.8651424
AACGCTA32300.024.7496723
CGCTACC33000.024.31406625
CTACACG37900.022.64938557
GCCCTAA38050.022.32778452
GTCCGTT12150.021.3713861
AAAACGC41500.021.18235421
CCTAAGC39750.021.1502554
CCCTAAG40100.021.03921153
GCCGGCT46550.020.7912431
CGAGCTA6350.020.44011511
CGAGGCG26700.020.43940454
TACACGT42300.020.2934258
GAAAACG44600.019.77617820
CCGGCTT55800.019.3495922
GTTCCTA28150.019.28170815
GCACTAC28250.019.10888125
GCCCCGT19050.018.8969251
ACACGTT46400.018.53830159
TCACGCT49200.018.5266868
Produced by FastQC (version 0.11.5)