FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21568121
Sequences flagged as poor quality0
Sequence length65
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTATGCCGTCTTCTGCTTG447730.2075887834642619TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG56800.049.9079548
CGTATGC57450.049.39462746
CTCGTAT57500.049.19765544
TCGTATG58900.048.27868345
GATCGGA59750.047.947461
TCTCGTA59300.047.75404743
ATCGGAA62150.046.2782822
ATGCCGT62900.045.20860349
ATCTCGT64450.043.8466342
ACACGTC65850.043.58637213
CGTCTGA66400.043.22533816
TGCCGTC66100.042.84147650
TCGGAAG71100.040.415923
GCCGTCT72150.039.41280751
ACGTCTG73800.039.21085715
CGTCTTC74750.037.81497253
CCGTCTT75550.037.52196552
CGGAAGA76450.037.510334
CACGTCT78100.037.08976414
GTATGCC80400.035.77247
Produced by FastQC (version 0.11.5)