FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27289687
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC806030.29536066133700983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG163450.032.9177745
ACCGCGT165000.032.608256
AGACCGC166700.032.523924
CAGACCG168800.032.2066733
CCGCGTT170000.031.6314837
CGCGTTC174350.030.893048
TCAGACC198550.028.7329062
AATACGG204850.026.62960632
CTCAGAC224500.026.5784991
GCGTTCT204200.026.4927469
TACGGAG205500.026.45923434
TTCGACG15100.026.372026
ATACGGA207550.026.32582733
CAATACG219150.025.33622431
GAACGAT219200.025.1099945
AACGATC224250.024.636646
AAGAACG226400.024.36355243
ACGGAGA224650.024.24314935
CGTTCTC232800.024.15034310
CGGAGAG228950.023.64382736
Produced by FastQC (version 0.11.5)