FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31942746
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTATGCCGTCTTCTGCTTG2457290.7692795102838059TruSeq Adapter, Index 25 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA277350.056.4110871
TATGCCG279950.055.91245348
CTCGTAT279700.055.7514944
CGTATGC282850.055.36005446
TCTCGTA282050.055.24513643
ATCGGAA284950.055.0405042
TCGTATG284800.054.9499445
ATGCCGT288050.054.26382449
ACACGTC290600.054.1226313
TGCCGTC291300.053.7292950
ATCTCGT290200.053.69413442
CGTCTGA292300.053.66758716
TCGGAAG293750.053.2116283
GCCGTCT293950.053.19732351
CGGAAGA296150.052.760484
ACGTCTG301500.052.02018415
CGTCTTC301150.051.9450653
CCGTCTT301950.051.81719652
CACGTCT307650.051.25737814
AATCTCG311900.050.1002441
Produced by FastQC (version 0.11.5)