FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11934067
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC537820.4506594440939539No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188160.1576662842600096No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT122840.1029322191671959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG14000.035.1852956
CAGACCG114400.034.640163
AGACCGC115700.034.1230434
ACCGCGT116700.033.5407526
GACCGCG117150.033.3478055
TCAGACC126950.032.774442
CCGCGTT120300.032.5358127
CGCGTTC120600.032.430428
CTCAGAC138550.031.2327481
GCGTTCT133950.029.2643329
GTTCGAC16800.029.0017645
TCGACGA16800.028.9688347
CGTTCTC146350.027.95386910
CGACGAG17200.027.6091988
CGGTATA24700.026.3917420
ATACGGA154950.024.89908833
TACGGAG153150.024.86410734
CCTCTCA165500.024.4526119
CAATACG160400.024.2554631
AATACGG161200.023.97050532
Produced by FastQC (version 0.11.5)