FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22688638
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGTATGCCGTCTTCTGCTTG255500.11261143132522983TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA39400.044.277031
TATGCCG40050.043.6022548
CGTATGC42000.041.78680846
CTCGTAT41800.041.63391544
TCTCGTA42650.040.59668443
ATGCCGT43800.039.7354549
TGCCGTC44100.039.39894550
TCGTATG45500.038.76692645
ATCGGAA45450.038.491182
GCCGTCT45250.038.39764451
ATCTCGT47100.036.63586842
CGTCTGA48700.036.46187616
ACACGTC49550.035.60346213
TCGGAAG49400.035.590253
CGTCTTC51050.034.15070353
CCGTCTT51700.033.66428452
CGGAAGA54900.031.80964
ACGTCTG56000.031.70881315
AATCTCG58400.029.7996241
CACGTCT59500.029.79401414
Produced by FastQC (version 0.11.5)