FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27230357
Sequences flagged as poor quality0
Sequence length65
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATCTCGTATGCCGTCTTCTGCTTG2706450.9939091140083107TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA296750.057.27661
TATGCCG297200.056.41642848
ACCGACT291700.056.2981232
ATCGGAA303850.055.974922
CACCGAC293900.055.95699731
CTCGTAT299250.055.9314844
CGTATGC300000.055.88014246
TCTCGTA300400.055.6977243
CCGACTG295100.055.62948633
CGACTGG294800.055.58603334
TCGGAAG306750.055.4720883
ATGCCGT302350.055.45547549
ACACGTC305850.055.4002713
GTCACCG301300.055.33655529
TGCCGTC303050.055.3273850
TCGTATG303950.055.25145
GCCGTCT304300.055.12918551
CGTCTGA306750.055.10339416
ATCTCGT305500.054.76790242
TCACCGA303600.054.6938630
Produced by FastQC (version 0.11.5)