FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22633423
Sequences flagged as poor quality0
Sequence length65
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTATGCCGTCTTCTGCTTG3894101.7205086477639728TruSeq Adapter, Index 10 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA551000.058.3267141
ATCGGAA551600.057.896932
TCGGAAG553350.057.5895083
CGGAAGA552700.057.064634
ACACGTC524700.057.057913
CGTCTGA529200.056.8184816
ACGTCTG534050.056.04839315
CACGTCT543000.056.00959414
GTCACGA540100.055.99950829
CTCGTAT556750.055.85543444
TATGCCG559700.055.84012648
ACGAGCT542450.055.8112932
CACGAGC539300.055.69421831
TCTCGTA558600.055.64932643
CGTATGC562300.055.60315346
AGTCACG541900.055.50779328
TCGTATG563850.055.48692745
TGCCGTC564150.055.43103450
GCCGTCT564950.055.34731751
GCACACG545600.055.34222811
Produced by FastQC (version 0.11.5)