FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27380432
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC671630.24529561841829228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC140100.031.2195594
GACCGCG141200.030.7671895
ACCGCGT144000.030.314946
CAGACCG146150.030.0486623
CCGCGTT149750.029.169937
CGCGTTC151700.028.7560848
TCAGACC179900.025.872672
TTCGACG20700.023.795986
CTCAGAC213200.023.5700861
GCGTTCT193350.022.6685319
CGTTCTC232800.019.61281410
AATACGG226600.019.57728832
TACGGAG227850.019.35337834
ATACGGA230600.019.25038333
CGGTATA35600.018.55924220
CAATACG247900.018.26392431
GAACGAT245650.018.11852345
GTTCGAC27200.018.0239095
AACGATC252400.017.80926346
ACGGAGA250000.017.69736935
Produced by FastQC (version 0.11.5)