FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21898699
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC1290990.5895281724270469No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA304540.1390676222363712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG262600.033.768765
AGACCGC266400.033.4753274
CAGACCG269700.033.1969833
ACCGCGT268600.033.157666
TTCGACG17300.032.9107676
CCGCGTT279650.032.0051357
CGCGTTC281850.031.7657818
TCAGACC296350.031.64512
CTCAGAC310600.030.93381
GCGTTCT312150.028.8335239
AATACGG329600.027.43210832
CGTTCTC344000.027.18439710
TACGGAG331350.027.10954334
ATACGGA334850.027.0020133
CAATACG346650.026.31262431
CCCAATA354800.025.88263129
CCTCTCA361600.025.7141219
ACGGAGA351200.025.535335
GAACGAT356100.025.49026745
AACGATC362150.025.23555246
Produced by FastQC (version 0.11.5)