FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25708230
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17800.024.52679348
GATCGGA18550.023.705371
CGTATGC19000.022.82243346
CTCGTAT20300.021.5062144
TCTCGTA20900.020.32424543
TCGTATG22350.019.66558845
ATCGGAA23700.019.5499062
ATGCCGT24400.018.37629149
ATCTCGT24950.017.26158142
CGTCTGA28250.016.08059716
ACACGTC28500.016.04298213
TCGGAAG30300.014.9946193
TGCCGTC30200.014.65171550
GCCGTCT34200.013.19682551
ACGTCTG35200.012.90554215
CCGTCTT37600.012.55296852
CGGAAGA37450.012.3680914
CATCTCG36000.012.12711341
CGTCTTC39100.012.07139653
CACGTCT39150.011.67879914
Produced by FastQC (version 0.11.5)