FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005800804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005800804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25637748
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC505690.1972443133460864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG108350.030.930075
AGACCGC110250.030.558064
ACCGCGT111000.030.2433856
CAGACCG115750.029.3099523
CCGCGTT114750.029.126397
CGCGTTC117300.028.5181358
TTCGACG18550.026.3982626
TCAGACC146000.024.469752
GCGTTCT149000.022.6085769
CTCAGAC170650.022.2115631
AATACGG153350.022.0454432
TACGGAG152900.021.97449534
ATACGGA154050.021.96364433
GAACGAT160750.020.97484645
AACGATC167600.020.3111946
TCGACGA24950.019.9813867
CAATACG172600.019.92854931
AAGAACG173650.019.62026243
CGTTCTC180400.019.49095310
ACGGAGA174350.019.37237435
Produced by FastQC (version 0.11.5)